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KMID : 0380020130280050303
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Korean Journal of Biotechnology and Bioengineering
2013 Volume.28 No. 5 p.303 ~ p.309
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The Extract from Lysimachia foenum-graecum Induces Apoptosis in MCF-7 Breast Cancer Cells
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Lee Se-Hee
Kim Guen-Tae
Kim Jong-Il
Lim Eun-Gyeong
Kim In-Seop
Kim Young-Min
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Abstract
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The extract from Lysimachia foenum-graecum (LFE) has been known to possess various instructive characters including anti-oxidant, anti-obesity, fungicidal activities. However, the accurate mechanism of those effects of LFE is not well known. In that respect, we evaluated the apoptotic effect and anti-cancer efficacy of extracts of LFE in MCF-7 breast cancer cells. In this study, we hypothesized that LFE may exert cancer cell apoptosis through regulating p53 and mitochondria-mediated apoptotic proteins. And this substance can generate ROS to cause free radical-induced apoptosis. Accordingly, the generation of ROS by LFE triggers the activation of p53 which are accompanied by pro-apoptotic protein activation and suppression of pro-survival proteins. We determined with MTT assay, flow cytometry for detection of intracellular ROS and Annexin V-PI staining, Western blotting. Consequently, our researches demonstrated that the treatment of LFE to breast cancer cells resulted in an activation of p53, Puma, Bax, cleaved-PARP and an inhibition of Bcl-2 expressions.
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KEYWORD
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MCF-7 breast cancer cells, Reactive oxygen species (ROS), p53, Apoptosis
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